LOCUS       pLV-[hsa-miR-155]        9119 bp    DNA     circular     20-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7040..7900
                     /label=Amp(R)
     promoter        8101..8641
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5645..5879
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6454..6909)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5951..6081
                     /label=SV40 pA
     misc_feature    6082..6295
                     /label=SV40 ori
     misc_feature    4966..5562
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3584
                     /label=intron
     CDS             3631..4956
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3374
                     /label=hsa-mir-155 precursor
BASE COUNT     2165 a      2359 c      2471 g      2124 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct tctctcttgc 
     3121 aggtggcaca aaccaggaag gggaaatctg tggtttaaat tctttatgcc tcatcctctg 
     3181 agtgctgaag gcttgctgta ggctgtatgc tgttaatgct aatcgtgata ggggtttttg 
     3241 cctccaactg actcctacat attagcatta acagtgtatg atgcctgtta ctagcattca 
     3301 catggaacaa attgctgccg tgggaggatg acaaagaagc atgagtcacc ctgctggata 
     3361 aacttagact tcaggctagc tcgagctttt ggagtacgtc gtctttaggt tggggggagg 
     3421 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt 
     3481 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc 
     3541 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaaaactac 
     3601 ccctctagag tcgagctacc ggtcgccacc atggtgagca agggcgagga ggataacatg 
     3661 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 
     3721 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 
     3781 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 
     3841 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 
     3901 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 
     3961 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc 
     4021 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 
     4081 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 
     4141 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 
     4201 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 
     4261 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 
     4321 atggacgagc tgtacaagga cccaccggtc gccaccatga ccgagtacaa gcccacggtg 
     4381 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 
     4441 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 
     4501 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 
     4561 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 
     4621 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 
     4681 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 
     4741 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 
     4801 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 
     4861 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc 
     4921 gcgacctggt gcatgacccg caagcccggt gcctgagcgg ccgcgtcgac aatcaacctc 
     4981 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 
     5041 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 
     5101 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 
     5161 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 
     5221 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 
     5281 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 
     5341 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 
     5401 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 
     5461 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 
     5521 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc 
     5581 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 
     5641 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 
     5701 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     5761 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 
     5821 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     5881 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 
     5941 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     6001 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     6061 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 
     6121 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     6181 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 
     6241 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 
     6301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
     6361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     6421 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 
     6481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     6541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     6601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     6661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     6721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     6781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 
     6841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 
     6901 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     6961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     7021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 
     7081 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 
     7141 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 
     7201 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 
     7261 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 
     7321 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 
     7381 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 
     7441 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 
     7501 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 
     7561 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 
     7621 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 
     7681 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 
     7741 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 
     7801 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 
     7861 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 
     7921 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 
     7981 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 
     8041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 
     8101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 
     8161 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 
     8221 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 
     8281 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 
     8341 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 
     8401 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 
     8461 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 
     8521 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 
     8581 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 
     8641 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 
     8701 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 
     8761 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 
     8821 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 
     8881 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 
     8941 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 
     9001 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 
     9061 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
